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The aim of this textbook is to train new researchers in analyzing high-throughput omics data by building fundamental skills instead of focusing on technology or platform-specific features that change every few years. The authors seek a balance between breadth and depth in the broad field. The book contains many real examples to illustrate the methodological concept and biological relevance. Computer lab materials (data and hands-on programming code) are included along with homework exercises to provide real-world data analysis experiences.